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Each 12-well plate was rearranged with 200 L Calcein-AM/PI double staining reagent (Saibaikang, HR0444, China)

KPV (Lysine-Proline-Valine) is a C-terminal fragment of -MSH studied in inflammatory-pathway research, with reported effects on NF-B signaling and cytokine modulation in cell-culture and animal models