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Higher alcohol concentrations risk denaturing sensitive peptide bonds and disrupting tertiary structure, which is why this exact ratio has become the laboratory standard for peptide reconstitution work over several decades
Cellular uptake of the tat protein from human immunodeficiency virus
An R ratio greater than 5 indicates hepatocellular injury, a ratio between 2 and 5 signifies mixed injury and a ratio less than 2 indicates cholestatic injury
Rafraf, M., Mohammadi, E., Asghari-Jafarabadi, M
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