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Amongst these mutations, 182 (83.9%) are single nucleotide substitutions, 19 (8.7%) are multiple nucleotide substitutions, 11 (5.1%) are deletions, and 5 (2.3%) are intronic mutations
After treatment, cells were immediately analyzed for apoptosis and the cell cycle using a BD FACSCelesta Cell Analyzer with an Annexin V-FITC/PI Apoptosis Detection Kit and PI/RNase Staining Buffer (BD Biosciences, Franklin Lakes, NJ, USA), respectively, according to the manufacturers instructions
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