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Pellets were resuspended in a volume equivalent to 10% of the initial culture volume with PBS containing 1% Triton X-100 and transferred into tubes containing glass beads (MP Biomedicals)
Most studies indicate it may only work on sun-exposed areas and in specific age groups over 40 years old
The compound's reputation rests on preclinical animal and in-vitro work, much of which is now compromised
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