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doi: 10.1128/MCB.01259-09 175 YangYChangBHSamsonSLLiMVChanL
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Local injection is typically placed in the subcutaneous tissue near the injured area, not directly into a tendon, ligament, nerve, blood vessel, or joint unless a specific procedural approach is being performed by a qualified clinician
After incubation with primary antibodies, the membranes were washed in TBS with 0.05% Tween (TBST) three times for 15 minutes and incubated with anti-goat, anti-rabbit, or anti-mouse HRP-linked secondary antibody at concentration 1:7,500 for 60 minutes at room temperature with gentle agitation
Bjrke-Monsen, Anne-Lise, and Per Magne Ueland
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