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based on consistent formation of -helical structure in initial rounds of structure calculations in which no hydrogen bond restraints were defined), inter-molecular PRE restraints (4 non-interacting copies of the spin label to account for different spin label orientations), inter- and intra-molecular NOE restraints, and ambiguous distance restraints for residues showing CSPs upon binding

Human clinical data remains limited for both forms

After the rats were anesthetized with pentobarbital (80 mg/kg i.p.), a laparotomy along the midline was performed and the stomach was fully exposed

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