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A broad panel of antibodies was employed, including those for FOXO4, p53, phospho-p53, p21, p16, cleaved-Caspase3, BAX, BCL-2, testosterone synthesis enzymes (3-HSD, CYP11A1, CYP17A1), and various SASP factors (IL-1, IL-6, TNF-, TGF-)

We compared postoperative inflammatory factor expression between groups and used PGE2, TNF-, and IL6 as markers of the anti-inflammatory effects of dexamethasone and VB12

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