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Lipidomic profiling of CE and TG species was performed by mass spectrometry-based analysis

For cytosolic folate variables and methionine cycle variables, see the discussions in our previous papers [1621]

Tier 3 (Recommended): Bone-age radiograph, IGF-1 and IGFBP-3 levels, thyroid function panel, and a validated depression/anxiety screen (PHQ-A for adolescents) before treatment initiation