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Description

Following a final saline wash, 50% glycerol was added to the wells, and bright-field images were taken using an inverted microscope

Ultimately, four studies were included

Cells were harvested, washed once with phosphate buffered saline (PBS, Gibco, #14190-094) and loosened from the cell flasks with Versene (Gibco, #15040-033) or TrypLE TM (Gibco, #12605-010)