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The following solutions were mixed together: 100 L of cells in TE buffer, 10 L of herring sperm DNA (10 mg mL 1 ), 10 L of SAT1 flipper disruption cassette (0.1 g L 1 ), and 5 L of histamine solution (Qbiogene)

LC-MS/MS was performed using an Evosep LC (Evosep Biosystems) connected to a Q Exactive HF (Thermo Fisher Scientific)

Many supplements are not suitable for certain dietary restrictions, including vegan and vegetarian diets

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Nausea and gastrointestinal symptoms peak during the first 1 to 2 weeks after this increase, then gradually improve as adaptation occurs