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Compared to NMR, MS offers high analytical power, allowing accurate and rapid quantification of labelling patterns, making it more suitable for flux measurements due to its widespread availability, lower cost, and sensitive detection of molecular enrichment (Antoniewicz, 2013b

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The 96-well assay plate media was then replaced with 180 l per well of freshly prepared assay media, and the plate was incubated at 37 C, no CO 2 , for 1 h to eliminate residues of carbonic acid