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A long term systemic infection of peach by Apple chlorotic leaf spot virus (ACLSV), the causal agent of viruela disease was investigated focusing on changes in host oxidative stress parameters and antioxidant capacity (Garca-Ibarra et al., Potato virus X (PVX)
Impurities such as truncated sequences or oxidation products can interfere with binding affinity assays, leading to skewed experimental outcomes
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However, no clinical trials validate efficacy in humans
An overnight culture was grown in LB-medium (1% tryptone, 1% NaCl, 0.5% yeast extract) from a freshly transformed single colony and was used to inoculate the expression batches
