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After 3 washes with PBS, cells were incubated with Alexa fluor 488 or 555 secondary antibodies (1:1000) (Thermo Fischer Scientific) as detailed in Supplementary Table S3 for 1 h at room temperature

The nasal mucosa is thin and highly vascularized, allowing relatively efficient absorption of peptides into the bloodstream

As an investigational agent, the full safety profile of retatrutide is still being characterised through ongoing clinical trials

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