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NTS prepared as previously described and cultured in M199 supplemented with 5.5 mM D-glucose, penicillin-streptomycin and 5% heat inactivated fetal bovine serum were incubated at 37 C in a clear bottom flat well plate in a 5% CO 2 incubator overnight
Then, the antioxidant glutathione also helps detoxify and regulate proper immune function, aids in cell repair and supports anti-aging and cognitive function
A 2016 study published in Nature Scientific Reports showed that GHK-Cu had no cytotoxicity to human keratinocytes at concentrations up to 5,800 micromolar over 72 hours and did not induce inflammatory biomarkers
However, given the normal rate of GSH efflux, a 48 hour half-life would require that catabolism replace 94% of daily dietary input, which seems improbably high
O., & Lerman, A
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