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Normally, FOXO4 binds to p53 in senescent cells (cells that no longer divide but are still active), stopping p53 from triggering apoptosis, or programmed cell death
Systematic substitution of each residue in the sequence with Ala enables the identification of critical amino acids governing protein bioactivity
Rational design using sequence information only produces a peptide that binds to the intrinsically disordered region of p53
Still, the comparison is instructive
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