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based on consistent formation of -helical structure in initial rounds of structure calculations in which no hydrogen bond restraints were defined), inter-molecular PRE restraints (4 non-interacting copies of the spin label to account for different spin label orientations), inter- and intra-molecular NOE restraints, and ambiguous distance restraints for residues showing CSPs upon binding
The pool turns over many times a day in active tissues
Then, to mimic the diabetic condition in vitro, we exogenously added glucose to the control medium, to obtain a final concentration of 35 mM
Oxidative medicine and cellular longevity, 2016, 4389410
Long-term deficiency can damage the protective myelin around nerves, and if it is not addressed, some of that damage can become permanent, causing numbness, pain, and balance problems
