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the combined washings and filtrate are washed either twice with water (1.15 kg each), or optionally with a sequence of water (1.15 kg), 5% aqueous sodium carbonate (3.38 kg), and twice with water (1.15 kg each)
Glutathione, often referred to as the "master antioxidant," is pivotal in maintaining mitochondrial integrity and function
However, the negative redox potential of roGFP2, the instantaneous nonenzymatic formation of roGFP2(S 2 ) from roGFP2(SSG), and the very slow nonenzymatic reaction of roGFP2(S 2 ) with GSH make the nonenzymatic shortcut from Fig
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