2.2 Laboratory-scale bioreactor cell culture At the laboratory scale, MRC-5 cells were cultured in 3 L glass bioreactors with a BioFlo 320 controller (Cat#: 1379962911, Eppendorf, Hamburg, Germany) on 1 g/L Cytodex 1 microcarriers (Cat#: 17548702, Cytiva, Marlborough, MA) or in iCELLis Nano fixed-bed bioreactors (Pall Corporation, Port Washington, NY) with a 10 cm bed height with WMEM and 10% BCS controlled at a target temperature of 37C and pH of 7.2 as described previously ( 250 HT reactors (Sartorius AG, Gttingen, Germany) with 1 g/L Cytodex 1 microcarriers were operated for oncolytic virus production similar to a published method ( 250 HT bioreactors underwent an 80% medium exchange into WMEM without serum for a final concentration of 2% (v/v) BCS
Nausea was the most frequent effect at 43.9% versus 16.1%
doi:10.1021/ci900316e [9] Burek CL, Talor MV
7A3), thus indicating that O 2 was generated in the reaction solution
doi: 10.7326/0003-4819-132-4-200002150-00004 223 ChakerLBaumgartnerCden ElzenWPIkramMABlumMRColletTHet al