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Suppresses M1-ATMs recruitment into epididymal WAT (eWAT) in diet-induced obese mice, attenuating cytokines (e.g., IL-6, tumor necrosis factor- (TNF-)) and chemokines (e.g., macrophage inflammatory protein-1 (MIP-1) and MCP-1)-mediated inflammatory cascades [174, 185]
We utilized Superscript III reverse transcriptase enzyme (Invitrogen) with random hexamer primers and 3 g of RNA from each sample to produce cDNA according to the manufacturers protocol
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Li H, Zhou L, Zhou W, Zhang X, Shang J, Feng X, et al
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