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Cells were passaged when they reached 7080% confluence, again maintaining 30 10 6 cells at each serial passage, with 5FU added in fresh media every 3 days
Howell, S
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Important overlapping endogenous molecular processes of IVD degeneration and facet joint osteoarthritis include extracellular matrix degeneration, inflammation, oxidative stress, apoptosis, senescence and reduced autophagy
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