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Park, MS et al (2010)

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2.14 Animal model and experimental protocol 2.14.1 Establishment of ischemia-reperfusion mouse model and drug administration protocol Mice (C57BL/6 strain) were housed under controlled environmental conditions with a 12-hour light/dark cycle and ad libitum access to standard rodent chow and water

Clean the skin with alcohol, let it dry, pinch a fold of skin, insert the needle, inject slowly, withdraw, and press for a few seconds

Structurally, Ipamorelin is a synthetic pentapeptide ( Aib-His-D-2-Nal-D-Phe-Lys-NH2 , 711.9 Da) designed by NovoNordisk in collaboration with Ferring in the mid-1990s as a selective tool for the GHS-R1a receptor (Growth Hormone Secretagogue Receptor type 1a)