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Arch Pharm Res 42:383392

Synthesis (1) 156g (1mol) of whey acid was dissolved in 2000mL of water, warmed up to 80, and then added 1.2mol of ammonia with a mass percentage concentration of 25% with stirring, and the reaction time was 1.5 hours at the above temperature, after the reaction was completed, concentrated, cooled down, analyzed the crystals, and filtered to obtain ammonium salt of orotate

Optimize first: High-percentage gel selection, membrane pore size, transfer time, methanol level, and prevention of over-transfer