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They influence immune signalling, blood vessel formation, and the production of collagen used in tendons, ligaments, and cartilage

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For the cytoplasm and muscle, the sections were then stained in Biebrich scarlet-acid fuchsin solution for 1015 min, again washed with distilled water and treated with phosphomolybdic-phosphotungstic acid solution for 1015 min, at this step, and for collagen staining, slides were transferred to aniline blue solution for 510 min and rinsed with water and 1% acetic acid