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Overexpressed proteins were purified on a Ni-NTA column (Cytiva) with an elution buffer containing 50 mM NaH 2 PO 4 , 300 mM NaCl, and 300 mM imidazole

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[1] Compounded medications are not subject to the same rigorous manufacturing standards and quality controls as FDA-approved drugs, which raises important considerations regarding consistency, sterility, and potency