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Briefly, the assay was performed in 500 l of 50 mM Tris-HCL, 0.1 mM EDTA, pH 7.4 containing 1 IU of glutathione-reductase (10105678001, Sigma-Aldrich), 0.5 mM GSH (G6529, Sigma-Aldrich), 0.12 mM nicotinamide adenine dinucleotide phosphate (NADPH), (N6505, Sigma-Aldrich), and 0.2 mM H 2 O 2 (95321, Sigma-Aldrich) as substrate

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They found instant posttraumatic MR signal changes in 60% of the intervertebral discs