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All animal procedures were approved by the institutional review board

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SURMOUNT OSA demonstrated after 52 weeks of treatment, participants who received tirzepatide had greater likelihood of remission or improvement to mild OSA as well as reduced apnea-hypopnea index (AHI) values, hypoxic burden, and systolic blood pressure

The flow-through was discarded, and then the filter unit was incubated with 40 L of 50 mM AmBic with trypsin (1/100 concentration of protein) overnight at 37 C