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225,226,227,228 Backbone modification The modification strategies used in first-generation NADs have focused on modification of the phosphate backbone, mainly using other types of groups to replace the non-bridging oxygen atoms in the phosphate backbone, such as phosphorothioate (PS), methyl phosphate, and boranophosphate

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The reducing activity of GPX4 in cell lysates was examined by monitoring the rate of NADPH oxidations using tert-butylhydroperoxide (t-BuOOH) as the substrate

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