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Antignac, J

For purification of His-tagged proteins, the supernatant was loaded onto a Ni-NTA column (GE Healthcare Life Sciences, PA) followed by washing with five column volumes of 20 mM imidazole and subsequent elution with 250 mM imidazole in the presence of 0.05% (w/v) DDM and 5% glycerol (w/v)

You can offset at least some of this muscle loss by prioritizing protein intake and strength training during treatment, but major weight loss always results in loss of some lean body mass, as every competitive bodybuilder will tell you, Quebbemann says