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In mice treated with amoxicillin or ciprofloxacin, depletion of Lactobacillus reuteri (Firmicutes) led to reduced brain and serum GABA, downregulation of GABA-A receptors, and diminished 5-HT and DA, neurochemical changes that coincided with anxiety- and depression-like behaviors (Bibi et al., 2025)

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Freeze-thaw cycles should be minimized for both, but the consequences of a freeze-thaw cycle tend to be more pronounced for GHK-Cu due to local pH excursions during ice formation, which can disrupt Cu coordination