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Patel notes
Although PACE can select against the cleavage of researcher-defined off-target substrates, it does not explicitly select against the cleavage of other off-target proteins that an evolving protease may acquire the ability to process during evolution
Awasthi YC et al
Furthermore, glutathione reductase is a flavoprotein, and flavoproteins generally have a high deuterium KIE, as has been confirmed experimentally for glutathione reductase (Vanoni et al., 1990)
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