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Immunoblots were performed using proteins from protoplasts co-expressing CRCK3-GFP with HopAI1-HA or LET7-HA with an -GFP antibody for CRCK3 and -HA antibody for HopAI1 or LET7
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But the dosing regime differs widely from one investigation to another based on the purpose, methodology, and design of the experiment
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