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Primary hippocampal neuron cultures are used to study Dihexa's proposed effects on dendritic spine density and synaptic protein expression, typically measuring morphological changes via fluorescence microscopy and quantifying synaptic marker expression (such as PSD-95 or synaptophysin) relative to untreated controls
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It also decreased TNF-, IL-6 in bronchoalveolar lavage fluid, and myeloperoxidase activity in lung extracts
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