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In the current study, we first show in a large multicenter cohort of primary malignant peripheral nerve sheath tumors and second in a series of primary and metastatic melanomas that complete loss of H3K27 trimethylation is not a reliable marker to distinguish malignant peripheral nerve sheath tumor from melanoma

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Proteins were transferred onto nitrocellulose membranes using a wet transfer apparatus (Bio-Rad) at 100 V for 1 h

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