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For the invasion assay, matrix gel (50 L/well, Servicebio) was pre-coated onto the lower chamber, and cells were seeded at a density of 5 10 5 cells/mL
Its application in the treatment of neurogenic and refractory ED will follow as part of an extended clinical development pathway (NCT05231083)
The reaction mixture consisted of 50 L of enzyme extract, 1.75 mL of 50 mM sodium phosphate buffer (pH 7.0), and 0.1 mL of 4% guaiacol
MAXIMUM FLAVANOL CONCENTRATION: Each serving delivers 1200mg total bioactive compounds (600mg flavanols + 600mg flavonoids), including 600mg of epicatechin - 10X more concentrated than standard cocoa powder
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