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426,427 In a low pH environment, fusogenic peptides undergo pH-dependent conformational changes to form -helices, which insert into the endosomal membrane, causing instability and decomposition of the membrane structure

The sequence of free roGFP2-iL and GRX1-roGFP2-iL were cloned into the BamHI and NcoI restriction sites of the protein expression vector pQE-30 (Qiagen, www.qiagen.com) for the production of a recombinant protein containing an N-terminal His 6 -tag

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