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Consistently, subsequent studies have further supported the pivotal roles of Hsp90 and AR signaling in regulating spermatogenic homeostasis and preserving male fertility (Tang et al., 2021)

Following differential centrifugation, the P2 microsomal membrane fraction was resuspended in cold buffer (50 mm Tris-HCl, 150 mm NaCl, 12.5 mm MgCl2, 0.2% BSA, and 10% glycerol, pH 7.4), supplemented with protease inhibitor cocktail and PhosSTOP phosphatase inhibitor cocktail, aliquoted, and stored at 80 C until use

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