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JS-K, a glutathione/glutathione S-transferase-activated nitric oxide releasing prodrug inhibits androgen receptor and WNT-signaling in prostate cancer cells

Proc Jpn Acad Ser B Phys Biol Sci 2010;86:588-610

Preclinical studies have explored GHK-Cu effects in cardiovascular applications, where the peptide demonstrates capacity to reduce atherosclerotic plaque formation in hyperlipidemic mice through anti-inflammatory effects and improved endothelial function

[8] On concentration: clinical studies generally use 1-3% in optimized delivery systems

Cells were lysed at 4 C in a radioimmunoprecipitation assay (RIPA) lysis buffer (Beyotime Biotechnology)