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GLP-1 receptors are found throughout the body, including in the pancreas, gastrointestinal tract, and certain brain regions involved in appetite regulation and glucose homeostasis
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The study utilized human embryonic kidney cells (HEK-293) transfected to express the melanocortin-4 receptor, providing direct evidence of PT-141s agonist activity at this receptor subtype through measurement of intracellular cAMP accumulation
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