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Nagler, A

doi:10.5021/ad.2016.28.4.438 Bobyntsev II, Chernysheva OI, Dolgintsev ME, Smakhtin MY, Belykh AE

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After the treatment period, the culture medium of each well was replaced by 0.2 ml of calcein AM solution (Sigma-Aldrich) at 2 M in phosphate buffer [PBS 1X] and the culture plates were put into the incubator for 30 minutes before fluorescence reading using a Synergy H1 microplate reader (Biotek, Winooski, VT, USA

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