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Qubit, Invitrogen), and monitored with a Qubit 3.0 Fluorometer (Invitrogen, Life Technologies, Carlsbad, CA, United States), yielding a dsDNA concentration in g/mL

Measurement of antioxidant enzymes: superoxide dismutase, catalase, and glutathione peroxidase levels The protocols to evaluate the levels of antioxidant enzymes are of key importance to know whether alterations in antioxidant enzymes are directly linked with the diseases incidence and development or just represent secondary symptoms occurring parallel with the diseases, or even consequence of the diseases

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In addition, the body's ability to synthesize glutathione decreases with age