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doi:10.1212/01.wnl.0000036903.22461.39 Neff BA, Staab JP, Eggers SD, et al

Good protocol design always includes bench-level standardization

Cytochrome c release assay Cells were fractionated to obtain cytoplasmic and mitochondria-enriched fractions using Dounce homogenization and differential centrifugation as per the Abcam cytochrome c release kit (ab65311)

I went through treatment for another five years