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Mice were anaesthetized using sodium pentobarbital at a dose of 180 mg kg 1 (Dolethal, Vetoquinone)

Asn240Lys produced the highest yield with 39 mg per liter of culture, while Asn240Lys/Leu244Arg produced the lowest amount of pure enzyme with 11 mg per liter of culture (Table 1)

Vegetarians and vegans may also experience B12 deficiency, because animal products are the most common source of dietary B12

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It promotes the growth of bones, cartilage, and muscle tissue, and it plays a key role in the repair and regeneration of tissues