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This model, known for producing a higher amount of ROS, was used to assess oxidative stress in mouse liver using the ROS probe dihydroethidium, as demonstrated in previous studies (Patel et al
Terminal deoxynucleotidyl transferase (Tdt, Tdt: reaction buffer = 1:4) was added to each section, coverslipped and incubated for 1 h
But more isnt always better overtraining can increase oxidative stress
The same formulation produced 210-400% increase in lymphocyte lysis after two weeks
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