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In a randomized trial of semaglutide 2.4 mg vs placebo for 1 year, 529 participants with HFpEF with a BMI of 30 kg/m2, there was a very large reduction in symptoms, improvement in exercise function in the GLP-1 drug assigned group
Step 3: C18 column was washed with Acetonitrile: Water and then equilibrated with aqueous ammonium acetate
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