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Beyond youth: Understanding CAR T cell fitness in the context of immunological aging
This process depends on: Vitamin B6 (P5P) as an essential enzyme cofactor Adequate availability of glycine and glutamate Sufficient cellular energy (ATP) Proper liver and mitochondrial function When this pathway is inefficient, glutathione production may be impaired even if homocysteine, B12, or folate levels appear normal
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A375 cells were cultured until 3050% confluence, then treated with a complete growth medium containing 0.1 M doxorubicin (Sigma D1515)
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