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CDC summarizes USP guidance: date an opened multi-dose vial at first puncture and discard it within 28 days unless the manufacturer states another opened-vial date. The dose count and 28-day window together require three vials for the 12-week protocol

Figure 5: Changes in tissue weight in lean mice and obese mice across the three different experimental treatment groups

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GHK and DNA: Resetting the Human Genome to Health