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At the end of the treatment, seedlings were transferred back to fresh liquid MS medium together with 10 M 5-ethynyl-2-deoxy-uridine (EdU) for 3 h, EdU revelation and DAPI counterstaining were performed following the kit instructions (Thermo Fisher Scientific, Waltham, MA, USA)
45,46 As shown in Figure 2 , GLP-1RAs penetrate the blood-brain barrier and directly activate GLP-1 receptors in the hypothalamus, especially within the ARH nucleus, a key center for appetite regulation
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