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Ubiquitin, a conserved 76-amino acid protein, is first activated in an ATP-dependent manner by E1 ubiquitin-activating enzymes, then transferred to E2 conjugating enzymes, and finally attached to target proteins by E3 ubiquitin ligases, which provide the critical element of substrate specificity (Fig
This synergy enables researchers to study peptide-mediated interactions across molecular, cellular, and tissue levelsfrom transcriptional modulation and protein signaling to cellular migration and tissue architecture formation
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