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SNP-specific F-statistics were calculated to evaluate the instrumental efficiency of SNPs with R 2 , exposure sample size, and the number of SNPs (Supplementary Table S1) ( Statistical analysis An inverse-variance weighted (IVW) analytical approach was applied to provide MR estimation and showed the largest statistical power among all MR methods by combining each Wald ratio of multiple SNPs ( 0

In this process, sperm pellets were resuspended in methanol/water, sonicated, and incubated for protein precipitation, followed by centrifugation (Paiva et al., 2015)

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