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Immunoblotting For immunoblotting analyses, cells or tissues were lysed in RIPA buffer containing the protease and phosphatase inhibitor cocktail, and the protein concentration was estimated using a BCA protein assay kit (Thermo Fisher Scientific)

doi: 10.1016/J.MICPATH.2022.105923 4 AminovR

When red blood cells die following vein treatment or due to severe varicose veins, haemoglobin is released into the tissues and breaks down into haemosiderin (iron)

This observation highlights the need for stratification methods in cancer to define ser/gly high cancers that can benefit from (neo)adjuvant sertraline treatment